Rao N. Gadiparthi, Ph.D., Associate Professor
Department of Physiology & Biophysics

Increased arterial smooth muscle cell mass and accumulation of oxidized lipids are pathophysiological characteristics of this vascular lesion Atherosclerosis. We believe that a change in the redox state of the arterial wall triggers these pathophysiological events. Therefore, the major interest of our laboratory is to study the regulation and signal transduction mechanisms of redox-sensitive gene expression in vascular smooth muscle cells and investigate their role in neointima formation in a rat carotid balloon injury model of restenosis. The major techniques used in the laboratories are Northern and Western blot analyses, nuclear run off assays, electrophoretic mobility shift assays, 2-D gel electrophoresis, high performance liquid chromatography, matrix-assisted laser desorption ionization-time of light analysis, phosphopeptide mapping, reporter gene assays and tissue culture.

Recent Publications

ATF-1 mediates protease activated receptor-1 (PAR-1) but not receptor tyrosine kinase-induced DNA synthesis in vascular smooth muscle cells. Ghosh SK, Gadiparthi L, Zeng ZZ, Bhanoori M, Tellez C, Bar-Eli M and Rao GN. J Biol Chem 277:21325-21331, 2002.

A potential role for nuclear factor of activated T cells in receptor tyrosine kinase and G protein-coupled receptor agonist-induced cell proliferation. Yellaturu CR, Ghosh SK, Rao RK, Jennings LK, Hassid A and Rao GN. Biochem J 368:183-190, 2002.

N-Ethylmaleimide inhibits platelet-derived growth factor BB-stimulated Akt phosphorylation via activation of protein phosphatase 2A. Yellaturu CR, Bhanoori M, Neeli I and Rao GN. J Biol Chem 277:40148-40155, 2002.

5(S)-Hydroxyeicosatetraenoic acid stimulates DNA synthesis in human microvascular endothelial cells via activation of Jak/STAT and PI3-kinase/Akt signaling leading to induction of expression of basic fibroblast growth factor-2. Zeng ZZ, Yellaturu CR, Neeli I and Rao GN. J Biol Chem 277:41213-41219, 2002.

Thiol alkylation inhibits the mitogenic effects of platelet-derived growth factor and renders it proapoptotic via activation of STATs and p53 and induction of expression of caspase1 and p21waf1/cip1. Bhanoori M, Yellaturu CR, Ghosh SK, Hassid A, Jennings LK and Rao GN. Oncogene 22:117-130, 2003.

Cytosolic phospholipase A2 is an effector of Janus-activated kinase/Signal Transducers and Activators of Transcription signaling and is involved in platelet-derived growth factor BB-induced growth in vascular smooth muscle cells. Yellaturu CR and Rao GN. J Biol Chem 278:9986-9992, 2003.

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