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Welcome to the Tissue Services Core -
Information for investigators:
If you need human tissue (fresh
frozen or formalin fixed paraffin embedded) for research then please
send us
a request for tissue.
In recent years, cancer research has
started to shift from using cell lines and animal models to human
tissues. Due to the change in the pattern of research, investigators
often request that pathologists provide human tissue that is to be
discarded. The Pathology department utilizes a small part of tissue
for diagnosis, the remaining tissue is stored for about three weeks
after release of report, and then the tissue is discarded. Through
the establishment of the tissue core facility, the tissue can be
stored in a manner suitable for future use by
investigators.
Tissue will be provided to the
investigators for research only. The tissue will not be sold. The
Tissue Services Core will charge processing and administrative cost
for the samples or services provided to the investigators. The TSC
advisory committee will decide the cost for processing and
administration.
There are three commonly used models
of tissue repositories: prospective tissue collection model,
tissue banking model, and combination of the above two
models.
In a prospective tissue collection
model, tissue is collected according to the need of the researcher.
If one researcher needs breast cancer tissue while another
researcher needs prostate cancer tissue, then the tissue services
core will collect breast and prostate cancer tissue and no other
tissue.
A tissue-banking model collects and
stores various types of tumor and non-tumor tissues. Whenever an
investigator needs the tissue the stored tissue can be given without
delay.
We are planning to collect tissue to
use a combination of both the methods. As per investigator's request
we will start collecting the tissue. The processing of the tissue
would be performed following a protocol approved by the
investigator. if an investigator is interested in performing
research on the tissue that we have already collected using standard
protocol, we will be providing the tissue to the investigator.
Procedure:
These are guidelines although
collection and storage of solid tissue or body fluids might differ
according to the need of the investigators.
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Procedure for
procurement, processing and storage of solid tissue
specimens:
Solid
tissue will be transported as soon as possible from the operating
room, on ice, to the pathology grossing room. A pathologist,
pathology assistant or resident pathologist will examine the tissue
for gross abnormalities. Tissue required for diagnosis is collected.
Remaining tissue that is not required for diagnosis is cut into
small aliquots for snap freezing in liquid nitrogen, frozen after
embedding in OCT, and stored in formalin.
- Procedure for procurement, processing and storage of body
fluids:
Specimens of blood, bone marrow,
urine and other biological fluids must be kept sterile at all times.
After assurance that the necessary requirements for diagnostic
testing have been met, the tissue samples are stored using a
standard protocol.
The request from an investigator for
tissue and/or body fluids will be submitted to the Tissue Services
Core. The written request will include the names, addresses and
telephone numbers of all the investigators, an abstract of the
proposed study including brief background, methods to be used and a
copy of relevant IRB approval. The request will also include number
and type of samples (tissue, serum, plasma etc) required. The
members of tissue resource committee will review the request in a
timely manner. If approved, the chair would inform the director of
the Tissue Services Core to release the required samples.
Return to the Tissue Services Core
information
page or send us
a request for tissue.
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