Molecular Resource Center

Real Time PCR

LightCycler®480 (LC480)

Universal Probe Library

The LightCycler® 480 Instrument is a rapid, thermal blockcycler with integrated real-time, online detection capabilities. Based on a comprehensive improvement of the Peltier-based block technology, the Instrument provides extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. The special arrangement of optical components in the LightCycler® 480 Instrument ensures the uniform collection of signals across the plate and makes analysis independent of the sample position on the plate.

The LightCycler® 480 System setup enables the use of all current probe formats (e.g., SYBR Green I, Hydrolysis Probes, HybProbe Probes) and provides the ideal solution for fast and precise qualitative or quantitative detection of nucleic acids, genotyping, and mutation analysis. The ability to freely combine five excitation and six emission filters permits analysis of signals from multiple dyes (e.g., LightCycler® Red 610, 640, LightCycler® CYAN 500, FAM, VIC, HEX, Cy5, and Fluorescein) in monocolor as well as in multiplex assays.