The University of Tennessee Health Science Center - Department of Comparative Medicine

Lethal or Toxic Challenge

This policy applies to all studies involving challenge with microorganisms or chemicals that could reasonably be expected to cause significant discomfort for some animals.

A. Generic Protocols

Generic protocols for toxicology or infectious disease studies will generally not be considered by the ACUC. Separate protocols for each agent, or at least related groups of agents, must be submitted to the Committee.

B. End Point of Lethal or Toxic Challenges

If the agent has the potential to cause significant discomfort, the earliest possible endpoint to the study must be defined and used. The protocol must justify and state, as clearly as possible, the endpoint to be used.

C. Number of Animals

Careful consideration must be given to number of animals required to accomplish the objectives of the study. The number anticipated to be used must be clearly indicated and justified in the protocol.

D. Teaching Protocols

Toxic or lethal challenges with the potential to cause discomfort are not be be given in connection with teaching protocols except on anesthetized animals that will not be allowed to recover consciousness. Use of a very limited number of animals for the purpose of preparing educational materials such as videotapes may be approved.